Title page for ETD etd-02062013-040043

Type of Document Master's Thesis
Author Bennett, Lisa Tracy
URN etd-02062013-040043
Title Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii
Degree Master of Science
Department Anaerobic Microbiology
Advisory Committee
Advisor Name Title
Dean, Dennis R. Committee Chair
Chen, Jiann-Shin Committee Member
Johnson, John L. Committee Member
Larson, Timothy J. Committee Member
Sitz, Thomas O. Committee Member
  • Klebsiella pneumoniae
Date of Defense 1989-12-15
Availability restricted

Nitrogenase-catalyzed nitrogen fixation is a biochemically and genetically complex process requiring the participation of a number of different nif (nitrogen fixation) gene products. The nifF (electron transport), nifA (nif gene regulation) and nifE (FeMo-cofactor biosynthesis) genes from Azotobacter vinelandii were genetically analyzed. The nucleotide sequence of the nifF gene, which encodes a flavodoxin, was determined. Specific mutation strains indicated that in A vinelandii flavodoxin is not the unique physiological electron donor to nitrogenase. The nifF gene appears to be constitutively expressed but under nitrogen fixing conditions nifF gene expression is stimulated.

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