Title page for ETD etd-02072013-040111

Type of Document Master's Thesis
Author Baruffaldi, Joan Marie
URN etd-02072013-040111
Title Adrenergic regulation of splenic functions in neonatal pigs
Degree Master of Science
Department Veterinary Medical Sciences
Advisory Committee
Advisor Name Title
Huber, William G. Committee Chair
Freeman, Larry E. Committee Member
Gwazdauskas, Francis C. Committee Member
Wilcke, Jeffrey R. Committee Member
  • Veterinary histology
Date of Defense 1989-04-05
Availability restricted
The purpose of this study was to assess adrenergic control of splenic

hemodynamic function and oxygen metabolism in neonatal pigs (NP).

Seventeen piglets, 28-45 days of age, were anesthetized with pentobarbitol (30

mg/kg, i.p.) and prepared for measurement of splenic venous outflow.

Simultaneous arterial and venous blood samples were analyzed for O2 content

and hematocrlt (Hct). Splenic oxygen consumption and extraction were

calculated. The effects of adrenergic stimulation on splenic leukocyte migration

and proliferation were also assessed. Fluorescence histochemistry of the

spleen from NP revealed noradrenergic innervation of the vasculature taken

from the hilar portions of the spleen. Norepinephrine (NE) Infusion ,

(2,ug/kg/min) caused a significant decrease in splenic venous outflow (P< 0.01)

with a concomitant significant increase in splenic resistance (P< 0.005). Splenic

leukocyte migration and proliferation did not change significantly during NE

infusion, but the splenic venous Hct was significantly increased (P< 0.001).

Similar changes were observed with electrical stimulation of the splenic nerve.

Pretreating the NP with beta-adrenoceptor blocker, propranolol (1 mg/kg), had

no significant effect on these responses. ln contrast, these responses were

abolished with the addition of alpha-adrenoceptor blocker, phentolamine (1

mg/kg). Splenic O2 metabolism did not change significantly during nerve

stimulation, but splenic venous Hct was significantly increased (P<

0re.spo0nses we5\re not altered by the adrenhoceptoreblockades. e

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