Title page for ETD etd-02132009-171134

Type of Document Master's Thesis
Author Aho, David W.
URN etd-02132009-171134
Title Influence of lipid antioxidants on soybean seed storage life
Degree Master of Science
Department Crop and Soil Environmental Sciences
Advisory Committee
Advisor Name Title
Parrish, David J. Committee Chair
Buss, Glenn R. Committee Member
Orcutt, David M. Committee Member
  • Soybean
Date of Defense 1991-05-05
Availability restricted

In-storage losses of seed vigor, i.e., aging, that occur between harvest and planting may leave soybeans essentially worthless as seed. Peroxidation of lipids, with resultant loss of membrane integrity, is theorized to be a primary event in seed aging. Lipid antioxidants might have the potential to protect dry seed by neutralizing free radicals, which propagate lipid peroxidation and other destructive events. Seed were treated with antioxidants in dimethyl sulfoxide (DMSO) to investigate possible protective effects of antioxidants during storage and thus also provide evidence for the lipid peroxidation model of seed aging.

The toxicity of DMSO to soybean seed was found to be minimal at treatment times of 15 min or less. Seed were treated for 15 min with 0, 5, 25, and 50 mM solutions in DMSO of propyl gallate (PG), butylated hydroxyanisore (BHA), butylated hydroxy toluene (BHT), and tert-butylhydroquinone (TBHQ). Germination, seed leakage, seedli,ng vigor, and phospholipids in the embryonic axis were monitored following storage at 40 C for up to 90 days.

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