Title page for ETD etd-02262009-055256

Type of Document Master's Thesis
Author Tyler, Carla
Author's Email Address catyler@vt.edu
URN etd-02262009-055256
Title Acceptability and Shelf-Life of Fresh and Pasteurized Crab Meat Stored Under Different Environmental Conditions.
Degree Master of Science
Department Food Science and Technology
Advisory Committee
Advisor Name Title
Jahncke, Michael L. Committee Chair
Sumner, Susan S. Committee Co-Chair
Boyer, Renee R. Committee Member
Hackney, Cameron Raj Committee Member
  • Listeria monocytogenes
  • packaging
  • shelf-life
  • crab meat
  • Carnobacterium piscicola
Date of Defense 2009-02-02
Availability restricted
Crab meat is important to the economy of coastal Virginia. The objectives of this study were to complete a shelf-life study on two different packaging styles of fresh crab meat and to test the inhibition capabilities of Carnobacterium piscicola against the pathogen, Listeria monocytogenes. In a shelf-life study, a 12 ounce food grade polyethylene traditional snap-lid container of fresh crab meat was compared to an 8 ounce SimpleStep® trays with Cryovac™ film of equally fresh crab meat sealed with 10,000 cc/m2/24hr oxygen transmission rate (OTR) film. Eleven g samples were used for the microbial shelf-life study conducted at 4oC for 12 days. Aerobic plate counts of crab meat indicated microbial growth from the SimpleStep® trays with Cryovac™ film in 10,000 cc/m2/24hr OTR versus the polyethylene snap-lid was not significant (P>0.05). In objective two, 25 g samples of fresh and pasteurized blue crab (Callinectes sapidus) meat were inoculated with 0.1ml of each, C. piscicola and L. monocytogenes. Three different concentrations of the inoculation levels were studied on select days at both 4oC and 10oC. Microbial spoilage was defined as 107 CFU/g. In fresh crab meat, at both 4oC and 10oC, crab meat spoilage occurred at 7 days or less. In the pasteurized crab meat, at 4oC and 10oC, spoilage did not occur prior to 26 days, and studies were terminated at 28 days of storage. The growth of the two organisms in fresh crab meat was found to be significant for the differing concentration levels and sampling days (P<0.05). The growth of the two organisms in pasteurized crab meat was significant for different concentration levels, sampling days and temperature (P<0.05). In both fresh and pasteurized crab meat, regardless of the inoculation ratios, the L. monocytogenes and C.piscicola followed similar growth trends, but L. monocytogenes was higher in the 2:2 CFU/g concentration and lower at the 6:2 CFU/g concentration level. Although C. piscicola did not completely inhibit L. monocytogenes growth at any concentration ratio, some inhibition was observed.
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