Title page for ETD etd-03122013-040259

Type of Document Master's Thesis
Author Sedano, Rodolfo Canseco
URN etd-03122013-040259
Title Effect of immunoglobulins on early bovine embryo development in vitro
Degree Master of Science
Department Dairy Science
Advisory Committee
Advisor Name Title
Gwazdauskas, Francis C. Committee Chair
Barnes, Michael A. Committee Member
Vinson, William E. Committee Member
White, John M. Committee Member
  • Veterinary embryology
Date of Defense 1985-11-05
Availability restricted
Bovine morulae (day 6: n=257) were obtained to evaluate

the [effect of immunoglobulins (Ig) on early bovine embryo

development in vitro. Fifty—four cows superovulations were

conducted in 36 cows with follicle stimulating hormone.

Embryos were collected by non-surgical procedures and

morphologically evaluated and randomly assigned to culture.

Embryos were cultured in Ham’s F-10 containing 10%

(6.4 mg/ml) steer serum (SS), 1% (.64 mg/ml) bovine gamma .

globulins (GG), 1% (.64 mg/ml) bovine IgG, 1% (.64 mg/ml)

bovine 1gM, 10% SS plus 1% GG, 10% SS plus 1% 1gG, or 10% SS

plus 1% 1gM. Embryos were cultured to the hatched blastocyst

lstage or degeneration and evaluated at 12 h intervals.

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