Title page for ETD etd-04042002-070623

Type of Document Master's Thesis
Author Sink, Carolyn
Author's Email Address csink@vt.edu
URN etd-04042002-070623
Title Canine Platelet Concentrates: An In Vitro Study to Effectively Provide a Source of Functional Platelets
Degree Master of Science
Department Veterinary Medical Sciences
Advisory Committee
Advisor Name Title
Feldman, Bernard F. Committee Chair
Monroe, William Edward Committee Member
Panciera, David L. Committee Member
  • canine platelet concentrate
  • storage lesion
  • blood component
Date of Defense 2002-03-25
Availability unrestricted
This study monitored the storage lesion of 15 units of canine platelet concentrates harvested by differential centrifugation. Canine platelet concentrates were stored at 20-24° C in a platelet rotator for a total of 9 days; the storage lesion of three second generation platelet storage containers was compared. The battery of in vitro tests used to monitor the storage lesion were selected from previous studies performed with human platelet concentrates separated by differential centrifugation. Based on these tests, canine platelet concentrates exhibited a storage lesion similar to human platelet concentrates. Metabolic analytes demonstrated decreasing pH, carbon dioxide, bicarbonate and glucose concentrations concurrent with increasing oxygen and lactate dehydrogenase activity over the 9-day period. Platelet structural changes were monitored by mean platelet volume, which began to increase on Day-5. Platelet function appeared to be compromised, as indicated by aggregation studies using collagen and adenosine diphosphate as agonists. Product sterility was maintained.

There was no consensus of data supporting superior performance of one platelet storage container. This study indicates that canine platelet concentrates may be harvested by differential centrifugation of whole blood. In vitro studies utilizing three second-generation platelet storage bags support a previous study and concurs that canine platelet concentrates stored at 20-24° C using continuous agitation are viable for at least 5 days.

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