Title page for ETD etd-04072010-020253

Type of Document Dissertation
Author Maciorowski, Anthony F.
URN etd-04072010-020253
Title The development and assessment of an automated biological monitoring system using crayfish locomotor activity to detect toxic stress.
Degree PhD
Department Zoology
Advisory Committee
Advisor Name Title
Benfield, Ernest Fredrick Committee Chair
Cairns, John Jr. Committee Member
Cochran, Donald G. Committee Member
Dickson, Kenneth L. Committee Member
Hendricks, Albert C. Committee Member
Patterson, Robert A. Committee Member
  • waste products
Date of Defense 1978-06-15
Availability restricted

In-plant biomonitors, controlled by dedicated microcomputers, may ultimately provide detection of developing toxicity and hazardous spills in industrial effluents rapidly enough to prevent serious damage to receiving waters. A computer automated system was developed to continuously monitor spontaneous locomotor activity of eight crayfish. Electric potentials generated by muscular movements of untethered crayfish were detected by external electrodes, amplified, digitized, and analyzed by a hybrid computer network. A microcomputer evaluated the number of peaks produced in the waveforms of eight individual C. acuminatus for successive one-hour intervals. The resultant counts were directly related to locomotor activity. The effectiveness of the crayfish biomonitor in detecting simulated spills of cadmium in the laboratory was determined by 7 experiments with 56 crayfish. Results indicated that crayfish responded to 2.5 and 0.5 mg Cd++/l with increased activity, which was detected by the crayfish biomonitor two hours after exposure. A simulated spill of O.1 mgCd++/l was detected by the biomonitor within 113 hours. These results support the assumption that the crayfish activity monitor may be used as a potential tool for water quality management.

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