Title page for ETD etd-05042006-164512

Type of Document Dissertation
Author Askew, David
URN etd-05042006-164512
Title Changes in macrophage functions and gene expression during tumor growth
Degree PhD
Department Biology
Advisory Committee
Advisor Name Title
Elgert, Klaus D. Committee Chair
Burger, Carol J. Committee Member
Nagarkatti, Prakash S. Committee Member
Rutherford, Charles L. Committee Member
Schurig, Gerhardt G. Committee Member
  • Tumors Growth
  • Gene expression
  • Macrophages
Date of Defense 1993-01-25
Availability restricted

Functions and phenotypes change in macrophages (Mø,) during tumor growth. Although analyzing functional and phenotypic changes are important in understanding the mechanism of tumor-induced immunosuppression, it is necessary to look beneath the surface and expose the mechanisms behind these changes. Flow cytometrically isolated Mac-1 +, -2+, or -3+ Mø, showed that although both normal host and tumor-bearing host (TBH) Mac-2+ Mø, were the primary source of prostaglandin E2, no specific TBH suppressor Mø, could be identified. To determine if normal host and TBH Mø, respond to in vitro activating agents differently, normal host and TBH Mø, were treated with lipopolysaccharide. Functional, phenotypic, and molecular changes were observed in the lipopolysaccharide-treated M4J. Three- and 24-h lipopolysaccharide treatment reduced TBH Mø,-mediated suppression, while only 24-h lipopolysaccharide treatment reduced it in normal host Mø,. Prolonged adherence, which induces Mø, differentiation, increases the number of Mac-2+ TBH Mø,. Tumor growth causes an increase of Mø, in the S and G2/M phases of the cell cycle. Lipopolysaccharide and adherence increase the number of normal host Mø, in Sand G2/M; however, these same treatments reduce the nu mber of TBH Mø, in these same phases.

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