Title page for ETD etd-06052008-100915

Type of Document Master's Thesis
Author Machen, John Wesley
Author's Email Address jmachen@vt.edu
URN etd-06052008-100915
Title Vibrio spp. disinfection and immunization of Cobia (Rachycentron canadum) for the prevention of disease in aquaculture facilities.
Degree Master of Science
Department Veterinary Medical Sciences
Advisory Committee
Advisor Name Title
Smith, Stephen A. Committee Chair
Flick, George J. Jr. Committee Member
Pierson, Frank William Committee Member
  • Rachycentron canadum
  • Cobia
  • Striped Bass
  • Morone
  • Vibrio
  • disinfection
  • disease
  • immunity
  • vaccination
Date of Defense 2008-05-22
Availability unrestricted
Cobia (Rachycentron canadum) is a tropical marine fish, with increasing commercial aquaculture importance worldwide. One of the major limitations to intensive aquaculture is disease. Diseases spread rapidly in an aquatic environment and pose a major threat to the development and introduction of new species, such as cobia, in aquaculture. This is due to the necessity to use wild caught broodstock, which pose a greater threat to introducing disease to a facility. Bacteria of the genus Vibrio play a major role in the diseases of cultured cobia and other species of marine fish. The goal of this study is to reduce the incidence of disease in a population, by either eliminating the potential pathogen or increasing the resistance of the host. To reach that goal, a disinfection assay to evaluate the effectiveness of nine common aquaculture chemical disinfecting compounds was evaluated against two bacterial pathogens (Vibrio anguillarum and V. ordalii). Both bacterial species were susceptible to a variety of common disinfecting compounds including Chloramine-T®, chlorine, ethanol, iodine, Lysol®, Roccal®-D Plus, and Virkon-S®. In addition, both species showed a resistance to disinfection with formalin and tap water. The humoral immune response of cobia to vaccination with a commercially-available vaccine for Vibrio spp. was evaluated by an ELISA. There was a significant difference between control and vaccinated groups (P<0.0001), showing significant antibody production resulting from vaccination.
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