Title page for ETD etd-06062008-171625

Type of Document Dissertation
Author Alber, Birgit E.
URN etd-06062008-171625
Title Carbonic anhydrase from Methanosarcina thermophila :proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolism
Degree PhD
Department Biochemistry and Anaerobic Microbiology
Advisory Committee
Advisor Name Title
James G, Ferry Committee Chair
Dean, Dennis R. Committee Member
Gregory, Eugene M. Committee Member
Johnson, John L. Committee Member
Niehaus, Walter G. Jr. Committee Member
  • carbonic anhydrase
  • CA
Date of Defense 1995-06-09
Availability restricted

Carbonic anhydrase (CA) from acetate-grown Methanosarcina thermophila strain TM-1 was purified> 10,OOO-fold (22% recovery) to apparent homogeneity and a specific activity of 4,900 units mg-1.The gene encoding this CA was isolated fronl a partial genomic library on a 12-kb fragment and sequenced. Comparison of the deduced anlino acid sequence with the N-terminaI sequence of the purified protein shows that the gene encodes an additional 34 N-terminal residues with properties characteristic of signal peptides in secretory proteins. The deduced amino acid sequence has no significant identity to any known CAs, but has, among others, 35% sequence identity to the first 197 deduced N-terminal amino acids of a proposed CO2-concentrating-mechanism protein from Synechococcus sp. strain PCC7942.

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