Title page for ETD etd-06102012-040255

Type of Document Master's Thesis
Author Shannon, Robert David
URN etd-06102012-040255
Title Evaluation of a mitochondrial test for the determination of chemical toxicity
Degree Master of Science
Department Environmental Sciences and Engineering
Advisory Committee
Advisor Name Title
Boardman, Gregory D. Committee Chair
Bevan, David R. Committee Member
Dietrich, Andrea M. Committee Member
Hoehn, Robert C. Committee Member
  • Toxicity testing.
Date of Defense 1988-07-15
Availability restricted
The feasibility of using rat liver mitochondria respiratory parameters

as a short-term toxicity test was investigated. Mitochondrial fractions were

exposed to six concentrations of five chlorophenols. Respiratory parameters

were measured and compared to control experiments. The toxicity of the

chlorophenos, measured by the 50% uncoupling concentration (UC50),

increased with increasing chloro substitution.

The UC50 values for the five chlorophenols were compared to six

physicochemical parameters for the same chlorophenols and high degrees of

correlation were found (r >/- 0.890). The highest correlation coefficient

obtained was with the octanol-water partition coefficient. UC50 values were

also compared to nine currently existing short-term toxicity tests. High

degrees of correlation were obtained with several of these tests, including

bacterial and fish bioassays.

From the results of these experiments, the measurement and use of

mitochondria respiratory parameters as a short-term toxicity test appears to

offer an alternative to currently used short-term toxicity tests, particularly

with chemicals having physicochemical characteristics similar to

mitochondria uncouplers.

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