Title page for ETD etd-07242002-214305

Type of Document Master's Thesis
Author Schuchert, Jennifer Ann
URN etd-07242002-214305
Title Detection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reaction
Degree Master of Science
Department Veterinary Medical Sciences
Advisory Committee
Advisor Name Title
Inzana, Thomas J. Committee Chair
Boyle, Stephen M. Committee Member
Sriranganathan, Nammalwar Committee Member
  • Multiplex PCR
  • serotyping
  • Actinobacillus pleuropneumoniae
Date of Defense 2002-06-26
Availability unrestricted
Traditional immunological assays used to serotype Actinobacillus pleuropneumoniae have been problematic due to cross- reactivity between serotypes, particularly serotypes 6 and 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and identify serotypes 1, 2, and 8. Primers specific to the conserved capsular polysaccharide export region of A. pleuropneumoniae serotype 5 amplified a 880 bp fragment in all serotypes excluding serotype 4 or a 489 bp DNA fragment in all serotypes including serotype 4. Primers specific to the capsular polysaccharide biosynthesis regions of A. pleuropneumoniae serotypes 1, 2, and 8 amplified a 1.6 kb, a 1.7 kb, and 970 bp fragment in the respective serotype. This PCR assay detects A. pleuropneumoniae and identifies serotypes 1, 2, and 8.
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