Title page for ETD etd-07242012-040202

Type of Document Master's Thesis
Author Murphy, Stephanie A.
URN etd-07242012-040202
Title Effects of selenium and vitamin B-6 on growth of chemically- induced transplanted tumors in BALB/c inbred mice
Degree Master of Science
Department Human Nutrition and Foods
Advisory Committee
Advisor Name Title
Driskell, Judy A. Committee Chair
Blodgett, Dennis J. Committee Member
Elgert, Klaus D. Committee Member
Ritchey, Sanford J. Committee Member
  • Selenium
Date of Defense 1989-03-30
Availability restricted
Male weanling inbred, mice were inoculated with

fibrosarcoma cells (hindquarter) originally produced by 2-methylcholanthrene. Before inoculation, mice were randomly divided into three groups of 24 and one of 12

(control). After a one week acclimation period, each group was fed a diet containing either suboptimal vitamin B-6, 0.5 mg/kg diet; adequate, 7.0 mg/kg diet; or excess, 100 mg/kg diet. Controls were fed the adequate vitamin. B-6 diet. Twenty-four hours after

tumor cell inoculation, a series of sodium selenite injections (0.5 μg/.10 mL) were given to half of each treatment group and all controls. Mice were sacrificed

two wk after tumor inoculation. Tumors were excised and weighed. Selenium-treated mice had significantly smaller tumors as compared to untreated mice regardless

of vitamin B-6 treatment. The smallest tumors were

found in the selenium—treated group maintained on

adequate B-6, while the largest tumors were developed

by mice on the excess B-6 diet without selenium treatments.

All groups had similar blood selenium levels as

measured by gas chromatography. Tumor selenium levels,

analyzed by atomic absorption, were significantly

higher for untreated groups than selenium—treated

groups (larger tumor size). The excess and adequate

vitamin B-6 selenium-treated groups had significantly

lower tumor selenium levels than the adequate vitamin

B-6 untreated group. Plasma pyridoxal phosphate

(concentrations) determined radiometrically and tumor

vitamin B-6 levels determined microbiologically, related directly to dietary treatments. Sodium selenite

injections and adequate vitamin B-6 diets reduced the

size of fibrosarcomas in BALB/c inbred mice.

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