Title page for ETD etd-07292013-082943

Type of Document Master's Thesis
Author Fultz, Stanley Wakefield
URN etd-07292013-082943
Title The Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoa
Degree Master of Science
Department Dairy Science
Advisory Committee
Advisor Name Title
Saacke, R. G. Committee Chair
Akers, R. Michael Committee Member
Keenan, T. W. Committee Member
Pearson, R. E. Committee Member
White, J. M. Committee Member
  • spermatoza viability
Date of Defense 1984-12-15
Availability unrestricted

A series of in vitro studies were conducted to evaluate the effect of poly-l-lysine concentration, molecular weight, and encapsulation temperature on the post encapsulation survivability of spermatozoa. Viability of spermatozoa encapsulated at 2012 C using four poly-l-lysine concentrations (.05%, .15%, .25%, and .35%) did not differ over the 8 h incubation period. However, the viability of each of the four treatments was lower than that of the unencapsulated control (p<.05 and p<.01; percentage motility and percentage intact acrosomes, respectively), indicating spermatozoal damage occurred during the encapsulation process. Capsule wall thickness and integrity for the .15%, .25%, and .35% concentrations were greater (p<.Ol) than that of the .05% capsules.

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