Title page for ETD etd-08232010-153840

Type of Document Dissertation
Author Bowerman, Peter Alexander
URN etd-08232010-153840
Title Exploring protein interactions and intracellular localization in regulating flavonoid metabolism
Degree PhD
Department Biology
Advisory Committee
Advisor Name Title
Winkel, Brenda S. J. Committee Chair
Grabau, Elizabeth A. Committee Member
Schubot, Florian D. Committee Member
Tholl, Dorothea B. C. Committee Member
  • protein localization
  • flavonoid
  • protein-protein interactions
Date of Defense 2010-08-02
Availability withheld
The organization of biological processes via protein-protein interactions and the subcellular localization of enzymes is believed to be fundamental to many aspects of metabolism. Although this organization has been demonstrated in several systems, the mechanisms by which it is established and regulated are still not well understood. The flavonoid biosynthetic pathway offers a unique system in which to study several important aspects of metabolism. Here we describe a novel toolset of mutant alleles within the flavonoid biosynthetic pathway. In addition, we discuss the use of several of these alleles together with a number of emerging technologies to probe the role of subcellular localization of chalcone synthase, the first committed flavonoid biosynthetic enzyme, on metabolic flux, and to characterize a novel chalcone synthase-interacting protein. The over-expression of this interacting protein induces novel phenotypes that are likely associated with the production or distribution of auxin. Further, interaction analyses between recombinant flavonoid biosynthetic enzymes point to the possibility that post-translational modifications play an important role in promoting interactions.
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