Title page for ETD etd-08262010-130942

Type of Document Master's Thesis
Author Kocabas, Evren
URN etd-08262010-130942
Title Identification of native co-factors of MshB and MCA from Mycobacterium species
Degree Master of Science
Department Biochemistry
Advisory Committee
Advisor Name Title
Hernick, Marcy Committee Chair
Helm, Richard Frederick Committee Member
Klemba, Michael Committee Member
  • Mycobacterium tuberculosis
  • metal-dependent deacetylase
  • MCA
  • MshB
  • iron
  • zinc
  • Mycobacterium smegmatis
Date of Defense 2010-08-11
Availability restricted
Mycothiol (MSH), a low-molecular- weight thiol, is a primary reducing agent and essential for the survival of mycobacteria. The full pathway of MSH biosynthesis and detoxification includes various promising drug targets. Several metalloenzymes are involved in this pathway, such as a deacetylase (MshB) and mycothiol S-conjugate amidase (MCA). MshB catalyzes the deacetylation of GlcNAc-Ins to form GlcN-Ins and acetate. Mycothiol S-conjugate amidase (MCA) cleaves the amide bond of mycothiol S-conjugates of various drugs and toxins. The identification of the native co-factor is critical for the design of potent and effective inhibitors. Therefore, in this study, we identified the possible native co-factors of MshB and MCA from M. smegmatis and M. tuberculosis.

To reach our aim, we used a pull-down method to rapidly purify halo-MshB and halo-MCA under anaerobic conditions. Our data indicates that the metal bound to MshB and MCA anaerobically purified from E. coli grown in minimal medium is mainly Fe(II), while proteins purified under aerobic conditions contain bound Zn (II) and Fe(II) that varies with the metal content of the medium. For a further clarification of the metal ion preferences of MshB and MCA, we determined the MshB and MCA affinity for Zn(II) to be in the picomolar range and Ms MshB affinity for Fe(II) in nanomolar range. These results indicate that MshB and MCA can be found bound with either iron or zinc and this is independent to their affinities for these metal ions.

  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
[VT] Kocabas_E_T_2010.pdf 828.47 Kb 00:03:50 00:01:58 00:01:43 00:00:51 00:00:04
[VT] indicates that a file or directory is accessible from the Virginia Tech campus network only.

Browse All Available ETDs by ( Author | Department )

dla home
etds imagebase journals news ereserve special collections
virgnia tech home contact dla university libraries

If you have questions or technical problems, please Contact DLA.