Title page for ETD etd-08312000-16210046

Type of Document Master's Thesis
Author Chandran, Jayanth Sankrit
URN etd-08312000-16210046
Title Characterization of proteins and tissue remodeling components in porcine aqueous humor
Degree Master of Science
Department Chemical Engineering
Advisory Committee
Advisor Name Title
Van Cott, Kevin E. Committee Chair
Forsten-Williams, Kimberly Committee Member
Goldstein, Aaron S. Committee Member
  • matrix metalloproteinase
  • relaxin
  • glaucoma
  • aqueous humor
Date of Defense 2000-08-22
Availability unrestricted
Connective tissue remodeling is an important area of study in biomedical engineering

with respect to cancer and wound healing. Tissue remodeling components may be

involved in the pathogenesis of open-angle glaucoma. Risk factors for open angle

glaucoma include increased intraocular pressure (IOP), male gender, and advanced age.

In a 1963 study, the hormone relaxin decreased IOP in the human eye through a

mechanism that may involve the up-regulation of tissue remodeling matrix

metalloproteinases (MMPs). The effects of age and gender on MMP and protein activity

in porcine aqueous humor were determined in this study to identify correlations existing

between MMP activity and glaucoma risk factors. Gelatin zymography identified MMPs

at 66 kD and approximately 105 kD. The concentration of the 66 kD band compared to

human MMP-2 standard was 0.22 ± 0.06 ng/ml for the adult female (AF) samples and

0.28 ± 0.04 ng/ml for the juvenile samples. This difference in concentration was

statistically significant (p < 0.05). The concentration of the protease migrating to 66 kD

was statistically independent of gender. Casein zymograms identified two non-MMP

proteinases at 51 kD and 80 kD. The average total protein concentration for all aqueous

humor samples was 2.54 ± 0.89 mg/ml. The mean IgG, transferrin, and albumin

concentrations for all aqueous humor samples was 11.4 ± 4.2 mg/ml, 17.11 ± 6.8 mg/ml,

and 78.0 ± 26.3 mg/ml respectively. Results from these experiments establish baseline

levels of MMP and protein activity, allowing for identification of potential changes

caused by relaxin in tissue culture studies.

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