Title page for ETD etd-10042006-143858

Type of Document Dissertation
Author Jack, Richard F.
URN etd-10042006-143858
Title Characterization and site-directed mutagenesis of NifU from Azotobacter vinelandii
Degree PhD
Department Biochemistry and Anaerobic Microbiology
Advisory Committee
Advisor Name Title
Dean, Dennis R. Committee Chair
Chen, Jiann-Shin Committee Member
Ferry, J. Greg Committee Member
Larson, Timothy J. Committee Member
Newton, William E. Committee Member
  • gene product
  • gene protein
Date of Defense 1995-10-05
Availability restricted
In order to elucidate the function of the nifU gene product in nitrogenase maturation in Azotobacter vinelandii. the gene product has been hyperexpressed in Escherichia coli and characterized by various biophysical techniques. Following the initial characterization, site-directed mutagenesis of conserved cysteinyl residues was performed in order to gain further insight into the structure/function relationship of NifU.

Both the Fe protein and the MoFe protein of nitrogenase require processing by additional nif genes including nifM (Fe protein), and nifE, N, B, H, V, and Q (MoFe protein). Two additional genes, nifU and nifS, are required for the maturation of both nitrogenase component proteins. It has been proposed that they may somehow be involved in metallocluster biosynthesis (Jacobson et al., 1989b). Our laboratory has determined that the nifS gene product (Nifs) is a pyridoxal-phosphate containing enzyme capable of catalyzing the desulfurization of L-cysteine and can provide the inorganic sulfide necessary for in vitro metallocluster biosynthesis of the Fe protein (Zheng, et al., 1993: Zheng, et al., 1994).

  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
[VT] LD5655.V856_1995.J335.pdf 8.15 Mb 00:37:43 00:19:24 00:16:58 00:08:29 00:00:43
[BTD] next to an author's name indicates that all files or directories associated with their ETD are accessible from the Virginia Tech campus network only.

Browse All Available ETDs by ( Author | Department )

dla home
etds imagebase journals news ereserve special collections
virgnia tech home contact dla university libraries

If you have questions or technical problems, please Contact DLA.