Title page for ETD etd-11152013-040105

Type of Document Master's Thesis
Author Neel, Jack Fagg
URN etd-11152013-040105
Title An investigation of the effect of certain environmental factors on the membrane filter procedure for bacteriological examination of water--Part II
Degree Master of Science
Department Sanitary Engineering
Advisory Committee
Advisor Name Title
John D. Eye Committee Chair
John A. Whittemore Committee Member
Louis A. Pardue Committee Member
Russell C. Brinker Committee Member
  • Water
Date of Defense 1956-05-05
Availability restricted

The purpose of the investigation was to determine if coliform organisms isolate on membrane filters can be incubated at a humidity below the point of saturation.

The investigation consisted of comparing the development of coliform organisms on membrane filters incubated at various controlled levels of humidity with the development of coliform organisms incubated in a saturated atmosphere. The effect of humidity on various types of differentiating media was considered simultaneously with the above investigation. Several suggested methods for the regulation of humidity were examined and evaluated and two new methods were developed.

The final results indicate that it is possible to incubate coliform organisms on membrane filters at all levels of humidity above 35 percent saturation. There was no significant difference noted in the results obtained on the four differentiating mediums: Two Step Endo, Single Step Endo, Hajna~Damon Endo and Dehydrated Schedule Nutrient.

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