Title page for ETD etd-11152013-040537

Type of Document Master's Thesis
Author McGonagle, Lynn
URN etd-11152013-040537
Title Characterization of a 4.0 kilobase plasmid from Pasteurella multocida
Degree Master of Science
Department Accounting and Information Systems
Advisory Committee
Advisor Name Title
N. Sriranganathan Committee Chair
G. R. Carter Committee Member
S. M. Boyle Committee Member
  • Avian influenza A virus
Date of Defense 1989-07-05
Availability restricted
A 4.0 Kb (2.64 Mdal) plasmid was isolated from a fowl cholera strain of Pasteurella multocida (the Larsen strain) by alkaline lysis and cesium chloride purification. The plasmid, designated pLAR—1, was characterized in terms of its size and restriction sites. The restriction patterns produced by fourteen endonucleases were used to

generate a restriction map. Five restriction enzymes

cleaved the plasmid at multiple sites. Two enzymes, Bgl II

and Sal I had unique sites on pLAR-l. Twelve of the fifty six strains of P. multocida surveyed contained plasmids of different sizes which hybridized to pLAR-l. Strains

containing homologous plasmids were variable in serotype, dermonecrotoxin production, and origin (both in terms of the host and locale). pLAR—1 did not encode any of the enzymes necessary for the biochemical pathways contained within the API-2OE strip or siderophore production. pLAR—l

was cloned into the BamH I site of pBR322. Resultant

clones were approximately 8.363 Kb in length, ampicillin resistant and tetracycline sensitive. The pLAR—l::pBR322 constructs were transformed into Escherichia coli DH5 alpha.

Transcription of pLAR—l was not detected using biotinylated pLAR-1 as a probe in a Northern analysis of mRNA extracted from the Larsen strain grown under nutrient rich conditions.

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