Title page for ETD etd-11212012-040107

Type of Document Master's Thesis
Author Byrne, Robert Duane
URN etd-11212012-040107
Title Assessment of dairy product quality by selective incubation and rapid techniques
Degree Master of Science
Department Food Science and Technology
Advisory Committee
Advisor Name Title
Bishop, J. Russell Committee Chair
Eigel, William N. III Committee Member
Jones, Gerald M. Committee Member
  • Milk
Date of Defense 1989-12-05
Availability restricted

A study was conducted to investigate the use of bacterial numbers and metabolites for their Value in predicting potential shelf-life of pasteurized fluid milk. The study consisted of two phases. In the first phase, chemical inhibitors were evaluated for their ability to allow growth of gram-negative psychrotrophic bacteria while inhibiting growth of gram-positive organisms. Crystal violet, benzalkonium chloride, a mixture of crystal violet and benzalkonium chloride, and alkylaryl sulfonate were tested. These were incubated with milk samples separately inoculated with gram-negative and gram-positive bacteria, at 2l°C for 18 hours. Alkylaryl sulfonate allowed growth of gram-positive organisms. Crystal violet inhibited gram-positive organisms but was not as effective as the mixture of crystal violet and benzalkonium chloride, The inhibitor of choice was benzalkonium chloride as it inhibited growth of all gram-positive organisms tested and allowed growth of the gram-negative psychrotrophic organisms.

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