Title page for ETD etd-12162009-020346

Type of Document Master's Thesis
Author Gibbons, John R.
URN etd-12162009-020346
Title Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
Degree Master of Science
Department Dairy Science
Advisory Committee
Advisor Name Title
Gwazdauskas, Francis C. Committee Chair
Beal, W. E. Committee Member
Pearson, Ronald E. Committee Member
Vinson, William E. Committee Member
  • Ovum.
Date of Defense 1994-05-15
Availability restricted

Three experiments were conducted to determine an efficient method of obtaining oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal oocyte recovery combined with in vitro maturation, fertilization, and culture (IVMlIVFIIVC) can produce pre-implantation stage bovine embryos and also supply a source of ova for gene microinjection. In Experiment 1, once- (IX) vs. twice-weekly (2X) oocyte recovery frequencies were compared. No differences in recovery per session were observed (IX = 6.8 vs. 2X = 6.3 oocytes/session; p>O.1 0). However, the 2X group generated more oocytes on a weekly basis (IX = 6.8 vs. 2X = 12.6 oocytes/week; pO.IO) In these experiments, twice-weekly follicular aspiration without exogenous FSH, was the best scheme of oocyte recovery. This program provided a source of consistent, high quality oocytes that responded favorable to the IVMIIVFIIVC system and microinjection.

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