Title page for ETD etd-41598-161051

Type of Document Master's Thesis
Author Boone, James Hunter M.S.
Author's Email Address jaboone2@vt.edu
URN etd-41598-161051
Title Evaluation of a Monoclonal-based EIA for the Detection of Giardia lamblia and the Identification of the Antigen
Degree Master of Science
Department Biology
Advisory Committee
Advisor Name Title
Falkinham, Joseph O. III Committee Chair
Krieg, Noel R. Committee Member
Wilkins, Tracy D. Committee Member
  • Antigen
  • Immunoassay
  • Giardia
Date of Defense 1998-05-05
Availability unrestricted

I. A number of commercial enzyme immunoassay (EIA) tests are available for the diagnosis of giardiasis. In a time of rising health-care costs, there is a need for diagnostic tests that are rapid, specific, sensitive and inexpensive. In the first phase of this study, I developed a monoclonal-based EIA, the GIARDIA TEST, with these qualities in mind. This assay's performance characteristics were determined by a comparison study using conventional ova and parasite examination, immunofluorescence antibody test (IFA) and other commercial EIA tests. Studies were done in-house at TechLab, Inc. and at various U.S. medical facilities. Results were statistically analyzed to determine sensitivity (ability of the assay to detect a positive result), specificity (amount of crossreactivity), predictive positive value (the confidence in a positive result), predictive negative value (the confidence in a negative result) and overall correlation with the reference assay.

II. There remain many questions to be answered about the various antigens produced by Giardia lamblia and how they can be utilized as diagnostic markers. In the second phase of this study, I identified and partially characterized the antigen (Ct7 Ag) that reacts with the Ct7 monoclonal antibody (MAb). This MAb is an IgM class mouse immunoglobin that is utilized by the GIARDIA TEST and by an immunofluorence antibody test (IFA) which detects Giardia cysts in water and feces. The results of this study will provide physicians and researchers with detailed information about the Ct7 Ag and why it is a useful marker for giardiasis.

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