Title page for ETD etd-06102012-040053
|Type of Document
||Gordon, Gale Ross
||The isolation and characterization of a growth factor in rumen fluid for a strain of Butyrivibrio
||Master of Science
|Moore, W. E. C.
|Fulghum, R. E.
|King, Kendall W.
|Kramer, Clyde Y.
|Date of Defense
One or more factors which occur in bovine rumen fluid stimulate
the growth of a strain of Butyrivibrio. The stimulating material is
heat stable, organic in nature and non-dialyzable. It cannot be
extracted from rumen fluid with lipid solvents and is retained in part
on anion and cation exchange resins. It can be eluted from the resins
with strong acid. It is stable to enzymatic hydrolysis by trypsin.
Granular mucin or bovine saliva will partially replace the stimulatory
activity. The part of the material which was not replaced by mucin did
not appear to be any compound that is commonly used to stimulate bacterial
growth. The presence of a possible inhibitor for the growth of a strain
of Butyrivibrio was demonstrated.
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