Title page for ETD etd-09072006-110318

Type of Document Master's Thesis
Author Wuenschel, Jr., Jeffrey Carl
Author's Email Address jwuensch@vt.edu
URN etd-09072006-110318
Title Effects of Feeding Supplemental Eicosapentanoic Acid and Docosahexanoic Acid to Beef Females on Reproductive Responses and Free Fatty Acids
Degree Master of Science
Department Animal and Poultry Sciences
Advisory Committee
Advisor Name Title
Hall, John B. Committee Chair
Beal, W. E. Committee Member
Greiner, Scott P. Committee Member
Knowlton, Katharine F. Committee Member
  • Prostaglandin
  • Nutrition
  • Reproduction
  • Beef Cattle
  • Fatty Acids
Date of Defense 2006-08-09
Availability unrestricted
The objective of this study was to determine the effects of dietary supplementation of eicosapentanoic (EPA) and docosahexanoic acids (DHA) on reproduction in beef females. In experiment 1, cows (n = 31) were individually fed rumen protected fish meal (FM) or no fish meal (C) supplements. Estrus was synchronized and ovulation induced on d 37. Ovarian follicular growth and diameter were determined by ultrasound on d 35 and d 37. Serum progesterone (P4) profiles were analyzed on d 37 through d 52. On d 52 cows were cannulated, primed with estradiol-17β at -240 min, and stimulated to release PGF2α by oxytocin injection at 0 min with blood sampled every 15 min from -30 min to 240 min. Supplement type did not affect (P > 0.05) follicular diameter, follicular growth or P4 concentrations. In cows fed FM, prostaglandin metabolite (PGFM) concentrations tended (P ≤ 0.10) to be reduced at 0, 30, and 60 min. In experiment 2, crossbred heifers (n = 214) received FM or C concentrates with corn silage from 30 d before estrous synchronization until 14 d after artificial insemination (AI). Serum fatty acid profiles were determined in five heifers from each group . Estrus detection and AI were conducted from d 37 through d 39. Dietary treatment increased (P < 0.05) EPA and DHA concentrations. Dietary treatment did not affect estrus response or AI conception rates and pregnancy rate. Supplementation of FM increased EPA and DHA concentrations but did not affect reproductive factors.
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