Title page for ETD etd-11092012-040320

Type of Document Master's Thesis
Author Young, Roderick Whiter
URN etd-11092012-040320
Title The amino acid composition of chinchilla fur in relation to the fur-chewing syndrome
Degree Master of Science
Department Biochemistry
Advisory Committee
Advisor Name Title
King, Kendall W. Committee Chair
Johnston, G. Burke Committee Member
Pardue, Louis A. Committee Member
Wilson, F. D. Committee Member
  • Fur
Date of Defense 1957-05-17
Availability restricted

Amino acids were determined in two types of chinchilla fur (chewed and normal), using three methods for analyses The methods included microbiological assays of Barton-Wright(5) and two column chromatography techniques of Moore and Stein (7, 8).

The data obtained from this investigation indicate that of the 16 amino acids analyzed, only one (lysine) was forum to be significantly lower in the chewed fur, as indicated by the "t" test. This difference between the normal and chewed fur was significant at the 2.5% 1evel. Also, arginine was lower and histidine was higher in the chewed fur than in the normal fur, but these difference: were not as conspicuous.

In general, the microbioligical analyses were more time consuming end less reproducible than the chromatographic analysis. With the exception of proline, glycine, and leucine, higher values were obtained fer the mine acids using the microbiological methods than with the column fractions. The greatest difficulty was encountered in analyzing the basic amino acids.

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