Type of Document Master's Thesis Author Neel, Jack Fagg URN etd-11152013-040105 Title An investigation of the effect of certain environmental factors on the membrane filter procedure for bacteriological examination of water--Part II Degree Master of Science Department Sanitary Engineering Advisory Committee
Advisor Name Title John D. Eye Committee Chair John A. Whittemore Committee Member Louis A. Pardue Committee Member Russell C. Brinker Committee Member Keywords
Date of Defense 1956-05-05 Availability restricted Abstract
The purpose of the investigation was to determine if coliform organisms isolate on membrane filters can be incubated at a humidity below the point of saturation.
The investigation consisted of comparing the development of coliform organisms on membrane filters incubated at various controlled levels of humidity with the development of coliform organisms incubated in a saturated atmosphere. The effect of humidity on various types of differentiating media was considered simultaneously with the above investigation. Several suggested methods for the regulation of humidity were examined and evaluated and two new methods were developed.
The final results indicate that it is possible to incubate coliform organisms on membrane filters at all levels of humidity above 35 percent saturation. There was no significant difference noted in the results obtained on the four differentiating mediums: Two Step Endo, Single Step Endo, Hajna~Damon Endo and Dehydrated Schedule Nutrient.
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