Type of Document Master's Thesis Author Gore, Mitchell Taylor URN etd-11152013-040323 Title Invitro protein synthesis and degradation and cathepsin D activity in the muscles of selenium-vitamin E deficient sheep Degree Master of Science Department Animal Science Advisory Committee
Advisor Name Title F. D. McCarthy Committee Chair D. R. Notter Committee Member K. E. Webb, Jr. Committee Member S. H. Umberger Committee Member Keywords
- Muscle proteins
Date of Defense 1985-08-05 Availability restricted AbstractA study was conducted with 21 Suffolk x (Dorset—Coop-worth) lambs to investigate the in vitro synthesis and degradation
capabilities and the involvement of lysosomal enzymes
in Se-E deficient ovine skeletal muscle. A randomized
block design was utilized in which the lambs were subjected to 3 treatments: 1) A basal NaOH treated 85% corn, 15% hay diet to which was added a Se-E deficient mineral mixture (-Se—E), 2) The basal diet and deficient mineral mixture as fed in treatment 1 with the addition of a casein encapulated fat supplement fed at 3g•kg-1 body weight (-Se-E+fat), and
3) The basal diet plus a Se-E supplemented mineral mixture (+Se+E). Three lambs were killed at the start of the trial
with the remainder of the lambs killed after being on the diets an average of 110 days. In vitro protein synthesis and degradation was measured on the semitendinosus and triceps
brachi muscles. The activity of the lysosmal enzyme cathepsin D was estimated in skeletal and heart muscle. The skeletal, cardiac, kidney and liver tissues were analyzed for Se content. In addition, plasma E and whole blood Se levels were determined.
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