Title page for ETD etd-11212012-040023
|Type of Document
||Comeaux, Jay Louis
||Transfer of plasmids by genetically-engineered Erwinia carotovora
||Master of Science
|Cairns, John Jr.
|Lacy, George H.
|Date of Defense
The ability of a genetically-engineered Erwirzia carotovora subsp. carotovora (Ecc)
strain to transfer recombinant chromosomal DNA or plasmids to wildtype Ecc or
Pseudomonas fluorescens was tested on filters, within soil microcosms, and in planta. Ecc was engineered by chromosomal insertion of a disarmed endo-pectate lyase gene marked with a 1.4kb DNA fragment conferring kanamycin resistance. Plasmids RPI and
pBR322 were introduced separately into engineered Ecc clones. These strains served as
donors in genetic transfer experiments. No transfer of the inserted kan marker or of
pBR322 was observed under any experimental condition. In filter matings, RPI was
transferred to wildtype Ecc at a frequency of 3.6 X 10-2 transconjugants per donor (TPD)
and to P. fluorescens at a frequency of 2.4 X 10-5 TPD. In matings conducted in potato tubers inoculated using sewing needles, the respective frequencies were 4.0 X 10-4 and 2.0
X 10-4, while matings on potato slices yielded frequencies of 4.7 X 10-2 and 2.3 X 10-2.
In soil microcosms, the maximum transfer frequencies observed were 2.3 X 10-3 and 8.4
X 10-5 TPD.
|| Approximate Download Time
| 28.8 Modem
|| 56K Modem
|| ISDN (64 Kb)
|| ISDN (128 Kb)
|| Higher-speed Access
next to an author's name indicates that all
files or directories associated with their ETD
are accessible from the Virginia Tech campus network only.
If you have questions or technical
problems, please Contact DLA.