Type of Document Dissertation Author Henderson, Douglas Brian URN etd-12152004-145307 Title The Development of a New Cloning Strategy for the Biosynthetic Production of Brush-Forming Poly(Amino Acids) Degree PhD Department Chemical Engineering Advisory Committee
Advisor Name Title Davis, Richey M. Committee Chair Ducker, William A. Committee Member Goldstein, Aaron S. Committee Member Van Cott, Kevin E. Committee Member Williams, Kimberly Forsten Committee Member Keywords
- recombinant DNA
- poly(amino acids)
Date of Defense 2004-12-10 Availability unrestricted AbstractThe design and discovery of new surface-active polymers that self-assemble on solid substrates to form brush layers will have a major impact on numerous applications. Through recombinant DNA technology, there exists the potential to harness a cell's protein synthesis machinery to produce a brush-forming poly(amino acid) (or PAA) with an exactly specified amino acid sequence, thus controlling the polymer's composition at a level unequaled by conventional organic polymer synthesis. The presented work demonstrates the cloning, expression, purification and characterization of de novo-designed PAA's designed to form brush layers on alumina surfaces. Using conventional recombinant DNA methods, the feasibility of producing a PAA consisting of a poly-glutamate block and a poly-proline block was demonstrated. However, the PAA design was limited by the inherent limitations of conventional cloning techniques.
We introduce here the development of a simple and versatile strategy for producing de novo-designed, high molecular weight PAA's using recombinant DNA technology. The basis of this strategy is that small DNA modules encoding for short PAA blocks can be easily inserted directly into a commercially available and unmodified expression vector. The insertions can be made repeatedly until the gene encodes for a polymer of desired molecular weight and composition. Thus, sequential modifications can be made to the PAA without having to re-start the gene assembly process from the beginning, thereby allowing for quick determination of how these changes affect polymer structure and function. The feasibility and simplicity of this method was shown during the production of a PAA, consisting of a long zwitterionic tail block and a short acidic anchor block, designed to form optimal brush layers on alumina surfaces. The success and flexibility of this method indicates that it can be applied for production of de novo-designed polypeptides in general. It is hoped that this method will contribute towards the rapid development of bio-inspired protein-based polymers for a variety of applications.
This dissertation also contains research that aimed to use phage display technology to develop a new liposome-based immunoassay against biological toxins. This work was part of a collaboration effort with the U.S. Department of Defense and Luna Innovations.
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28.8 Modem 56K Modem ISDN (64 Kb) ISDN (128 Kb) Higher-speed Access 01Title_page.pdf 48.83 Kb 00:00:13 00:00:06 00:00:06 00:00:03 < 00:00:01 02Abstr_TOC_Fig_Tab_Abbr.pdf 190.38 Kb 00:00:52 00:00:27 00:00:23 00:00:11 00:00:01 03Chapt_1_Project_Summary.pdf 129.82 Kb 00:00:36 00:00:18 00:00:16 00:00:08 < 00:00:01 04Chapt_2_Backgound.pdf 405.23 Kb 00:01:52 00:00:57 00:00:50 00:00:25 00:00:02 05Chapt_3_1st_Generation_PAA.pdf 508.52 Kb 00:02:21 00:01:12 00:01:03 00:00:31 00:00:02 06Chapt_4_A_Brush-Forming_PAA.pdf 486.20 Kb 00:02:15 00:01:09 00:01:00 00:00:30 00:00:02 07Chapt_5_Development_of_New_Cloning_Strategy.pdf 356.19 Kb 00:01:38 00:00:50 00:00:44 00:00:22 00:00:01 08Chapt_6_Application_of_New_Cloning_Strategy.pdf 518.13 Kb 00:02:23 00:01:14 00:01:04 00:00:32 00:00:02 09Chapt_7_Conclusions_and_Future_Work.pdf 150.10 Kb 00:00:41 00:00:21 00:00:18 00:00:09 < 00:00:01 10Chapt_8_Phage_Display_Work.pdf 256.19 Kb 00:01:11 00:00:36 00:00:32 00:00:16 00:00:01 11Appendix_A_Cloning_Method_Manuscript.pdf 479.42 Kb 00:02:13 00:01:08 00:00:59 00:00:29 00:00:02 12Vita.pdf 42.35 Kb 00:00:11 00:00:06 00:00:05 00:00:02 < 00:00:01
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