Title page for ETD etd-12162009-020346
|Type of Document
||Gibbons, John R.
||Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection
||Master of Science
|Gwazdauskas, Francis C.
|Beal, W. E.
|Pearson, Ronald E.
|Vinson, William E.
|Date of Defense
Three experiments were conducted to determine an efficient method of obtaining
oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal
oocyte recovery combined with in vitro maturation, fertilization, and culture
(IVMlIVFIIVC) can produce pre-implantation stage bovine embryos and also supply a
source of ova for gene microinjection. In Experiment 1, once- (IX) vs. twice-weekly
(2X) oocyte recovery frequencies were compared. No differences in recovery per session
were observed (IX = 6.8 vs. 2X = 6.3 oocytes/session; p>O.1 0). However, the 2X group
generated more oocytes on a weekly basis (IX = 6.8 vs. 2X = 12.6 oocytes/week;
pO.IO) In these
experiments, twice-weekly follicular aspiration without exogenous FSH, was the best
scheme of oocyte recovery. This program provided a source of consistent, high quality
oocytes that responded favorable to the IVMIIVFIIVC system and microinjection.
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