Communications Project

Document Type:Master's Thesis
Name:Rebecca J. Cutright
Title:Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families
Degree:Master of Science
Committee Chair: Richard E. Veilleux
Committee Members:Eric Beers
Carl Griffey
Keywords:Potato, Haploid-inducing Pollinator, Anther Culture
Date of defense:July 16, 1998
Availability:Release the entire work for Virginia Tech access only.
After one year release worldwide only with written permission of the student and the advisory committee chair.


Monopoloid potato (2n = 1x = 12) can be derived either paternally through anther/microspore culture or maternally through crossing with a haploid-inducing pollinator. Evidence from other genera suggests that androgenic and gynogenic haploid populations derived from the same parent may differ due to gametic selection and/or epigenetic factors. Our objectives were to derive androgenic and gynogenic monoploid populations from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja (PP5 and BARD1-3). A total of 185 fruit was obtained from PP5 and 398 from BARD1-3, resulting from 85% and 65% fruit set, respectively. Seed lacking the dominant embryo spot marker carried by IVP101 were selected and germinated in vitro. From 29,300 PP5 IVP101 seeds, 278 were spotless, resulting in 27 monoploids. Approximately 37 monoploids were obtained from the 99,500 BARD1-3 IVP101 seeds of which 500 were spotless. In anther culture, PP5 and BARD1-3 yielded 0.16 and 1.67 embryos per anther respectively of which 51% and 44% of the regenerants were monoploid. A total of 32 anther-derived monoploids has been obtained from PP5 and 130 from BARD1-3. Rooted cuttings of 21 androgenic and 21 gynogenic PP5 monoploids were established in a greenhouse in each of three randomized complete blocks. Although the anther-derived monoploids appeared more vigorous, none of the differences between the two populations were significant. Flow cytometry revealed that anther derived progeny of PP5 and BARD1-3 had 3-8% greater nuclear DNA content than gynogenic monoploids derived from the same parental clones.

List of Attached Files

ch1.pdf ch1_figs.pdf ch2.pdf
ch2_figs.pdf ch2_tbls.pdf ch3.pdf
ch3_figs.pdf ch3_tbls.pdf intro.pdf

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